Phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver.
نویسندگان
چکیده
Rat liver phospholipids were radioactively labeled in vivo before purification of UDP-glucuronyltransferase to homogeneity. The pure enzyme contained very little phospholipid (approx. 0.7 mol of phospholipid/mol of protein). The solubilization detergent Lubrol 12A9 appeared to act as a phospholipid substitute, capable of supporting UDP-glucuronyltransferase activity. Phospholipase C did not inhibit the pure enzyme activity and pure UDP-glucuronyltransferase was stimulated by 40--100% by the addition of phospholipid dispersions.
منابع مشابه
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عنوان ژورنال:
- The Biochemical journal
دوره 171 3 شماره
صفحات -
تاریخ انتشار 1978